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tsv antiserum as  (DSMZ)
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DSMZ tsv antiserum as
Figure 2. Validation of the small RNA HTS. (a) Primers were designed based on the <t>Ilarvirus-genus-</t> specific contig sequences able to amplify most of the each of three genomic RNAs. (b) Agarose gel electrophoresis of the RT-PCR productsobtainedwith the primers shown in (a). M is the GeneRuler 100 bp Plus DNA ladder (Thermo Fisher Scientific, Waltham, MA, USA).
Tsv Antiserum As, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Validation of the small RNA HTS. (a) Primers were designed based on the Ilarvirus-genus- specific contig sequences able to amplify most of the each of three genomic RNAs. (b) Agarose gel electrophoresis of the RT-PCR productsobtainedwith the primers shown in (a). M is the GeneRuler 100 bp Plus DNA ladder (Thermo Fisher Scientific, Waltham, MA, USA).

Journal: Viruses

Article Title: Clematis vitalba Is a Natural Host of the Novel Ilarvirus, Prunus Virus I.

doi: 10.3390/v15091964

Figure Lengend Snippet: Figure 2. Validation of the small RNA HTS. (a) Primers were designed based on the Ilarvirus-genus- specific contig sequences able to amplify most of the each of three genomic RNAs. (b) Agarose gel electrophoresis of the RT-PCR productsobtainedwith the primers shown in (a). M is the GeneRuler 100 bp Plus DNA ladder (Thermo Fisher Scientific, Waltham, MA, USA).

Article Snippet: The PV-0309 isolate was tested with DAS–ELISA with the TSV antiserum AS-0903 available at DSMZ, which was raised against a TSV isolate originating from a Vicia faba sample from Sudan (PV-0903).

Techniques: Biomarker Discovery, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction

Figure 5. Phylogenetic analysis of the nucleic acid sequences of RNA1 of PrVI and other ilarviruses belonging to group 1. Viruses are referred by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Journal: Viruses

Article Title: Clematis vitalba Is a Natural Host of the Novel Ilarvirus, Prunus Virus I.

doi: 10.3390/v15091964

Figure Lengend Snippet: Figure 5. Phylogenetic analysis of the nucleic acid sequences of RNA1 of PrVI and other ilarviruses belonging to group 1. Viruses are referred by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Article Snippet: The PV-0309 isolate was tested with DAS–ELISA with the TSV antiserum AS-0903 available at DSMZ, which was raised against a TSV isolate originating from a Vicia faba sample from Sudan (PV-0903).

Techniques: Virus, Sequencing

Figure 6. Phylogenetic analysis of the nucleic acid sequences of RNA2 of PrVI and other ilarviruses belonging to group 1. Viruses are referred by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Journal: Viruses

Article Title: Clematis vitalba Is a Natural Host of the Novel Ilarvirus, Prunus Virus I.

doi: 10.3390/v15091964

Figure Lengend Snippet: Figure 6. Phylogenetic analysis of the nucleic acid sequences of RNA2 of PrVI and other ilarviruses belonging to group 1. Viruses are referred by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Article Snippet: The PV-0309 isolate was tested with DAS–ELISA with the TSV antiserum AS-0903 available at DSMZ, which was raised against a TSV isolate originating from a Vicia faba sample from Sudan (PV-0903).

Techniques: Virus, Sequencing

Figure 7. Phylogenetic analysis of the nucleic acid sequences of RNA3 of PrVI and other ilarviruses belonging to group 1. Viruses are referred to by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Journal: Viruses

Article Title: Clematis vitalba Is a Natural Host of the Novel Ilarvirus, Prunus Virus I.

doi: 10.3390/v15091964

Figure Lengend Snippet: Figure 7. Phylogenetic analysis of the nucleic acid sequences of RNA3 of PrVI and other ilarviruses belonging to group 1. Viruses are referred to by the GenBank accession numbers of the reference genomes, following by the full name of the virus. In case of PrVI only the abbreviated PrVI is displayed followed by the host name and the origin of the country (SLO—Slovenia, SK—Slovakia, GR—Greece, HR—Croatia, RU—Russia and HU—Hungary). The red sign indicates a new virus with a reference of which sequence was not included in the phylogenetical analysis in the original description of the virus [14].

Article Snippet: The PV-0309 isolate was tested with DAS–ELISA with the TSV antiserum AS-0903 available at DSMZ, which was raised against a TSV isolate originating from a Vicia faba sample from Sudan (PV-0903).

Techniques: Virus, Sequencing